Program Overview
The report organizes three active-preservation routes for university trials and follow-up validation: MR-I removes oxygen, MR-II removes oxygen while releasing carbon dioxide, and a compound route adds a sterilization function to the active modified-atmosphere system.
Challenge & Protection Requirements
The study must separate gas-control performance from the final freshness conclusion. Beef color, odor, purge, pH, total plate count, volatile basic nitrogen, initial freshness, barrier performance, seal quality, and cold-chain fluctuations all affect the result.
Solution
Use an untreated control together with a 504BZ / MR-I oxygen-absorption group, a 504EG / MR-II oxygen-absorption and carbon-dioxide-release group, and a compound preservation group. Record gas concentration at 0, 1, 3, 5, 7, 12, and 24 hours, then continue sensory and microbiological observations during storage.
Application Media


Products Used
504BZ / MR-I Oxygen Absorber
Oxygen-absorption-only route used for the documented low-temperature gas-reduction curve.
504EG / MR-II Oxygen Absorber + CO2 Release
Active modified-atmosphere trial route combining oxygen reduction with carbon-dioxide release.
Compound Preservation Route
The source report identifies this as the route with an added sterilization function but does not list a specific formula, format, or capacity.
MR-I / 504BZ Oxygen Reduction at 0–4 °C
| Time | MR-I O2 | MR-II O2 | MR-II CO2 | 504G CO2 (Reference) |
|---|---|---|---|---|
| 0 h | 21.00% | 20.90% | 0.00% | 0.00% |
| 1 h | 10.80% | 20.30% | 7.42% | 4.21% |
| 3 h | 5.10% | 18.80% | 13.40% | 11.40% |
| 5 h | 3.43% | 16.90% | 16.30% | 15.30% |
| 7 h | 1.40% | 15.40% | 19.20% | 17.80% |
| 12 h | 0.02% | 12.70% | 23.60% | 21.50% |
| 24 h | 0.01% | 7.51% | 29.40% | 26.30% |
Additional Validation Data
Three Trial Routes
| Trial Route | Reference Model | Primary Observation |
|---|---|---|
| Oxygen absorption only | 504BZ / MR-I | Rapid O2 reduction and low-oxygen stability |
| O2 absorption + CO2 release | 504EG / MR-II | Coordinated O2 reduction and CO2 increase |
| Compound preservation | Specific formula, format, and capacity are not listed in the source report | Gas change, total plate count, and sensory indicators |
Key Advantages
- Three Comparable Routes — Separates oxygen control, active modified atmosphere, and compound preservation.
- Measured Gas Curves — Uses seven documented time points from 0 to 24 hours.
- Representative Test Pack — Defines beef mass, tray dimensions, and chilled storage temperature.
- University Trial Ready — Includes control groups and recommended follow-up indicators.
- Evidence Boundary Preserved — Does not equate gas curves alone with a final preservation claim.